Proline Specific Endopeptidase from Flavobacterium sp.

Description

SPECIFICATIONS:

EC 3.4.21.26

Product name: Prolyl endopeptidase

Appearance: white amorphous powder lyophilized

Activity: Grade I, 5.0 U/mg-solid or more

Contaminants:

• Trypsin-like activity ≤ 0.1%
• Leucine aminopeptidase ≤ 0.1%

Stability: Stable at – 20 °C for at least 12 months

Molecular weight: Approx. 78,000

Structure: Monomer

Isoelectric point 9.1

Michaelis constants:

• 2.5×10-5 M (Z-Gly-Pro-MCA),
• 1.4×10-4 M (Z-Gly-Pro-2NNap)

Inhibitors: DFP, 3, 4-dichloroisocoumarin, Z-Gly-Pro-CH2Cl

Optimum pH: 6.5

Optimum temperature: 37℃ (40℃)

pH Stability: 5.5 – 8.5 (30℃, 15hr)

Thermal stability: Below 40 ℃ (pH 7.0, 10min)

Substrate specificity: Y-Pro (Ala)-X (Y, peptide or N-protected amino acid; X,
amino acid, peptide, amide, or ester)