Description
SPECIFICATIONS:
EC 1.1.3.22
Product name: Xanthine: oxygen oxidoreductase
Appearance: Reddish brown amorphous powder, lyophilized
Activity: Grade II, 10 U/mg-solid or more
Contaminants:
- Catalase: ≤ 5.0%, Adenosine deaminase: ≤ 1.0×10-3%
- Uricase ≤ 1.0×10-3%, Phosphatase ≤ 1.0×10-3%,
- Purine-nucleoside phosphorylase ≤ 5.0×10-3%
Stabilizers: Sodium glutamate, BSA
Stability: Stable at – 20 °C for at least 12 months
Molecular weight: approx. 160,000
Isoelectric point: 4.0 + 0.1
Michaelis constant: 4.5×10-5M (Xanthine), 7.6×10-5M (Hypoxanthine)
Inhibitors: Reducing agents, Hg2+, Ag+, MIA
Optimum pH: 7.5 – 8.0
Optimum temperature: 65 ℃
pH Stability: pH 6.5 – 9.0 (25℃, 15hr)
Thermal stability: below 55℃ (pH 8.0, 30min)