Urease from Jack Bean >100 U/mg

Urease from Jack bean

Product Code: 174591

EC no:     3.5.1.5

Product Code: 174591 Category:

Description

SPECIFICATIONS:

EC 3.5.1.5

Product name: Urea amidohydrolase

Appearance: white amorphous powder lyophilized

Activity: Grade II, 100U/mg-solid or more

Contaminants:

  • Asparaginase < 2.0×10-2 %,
  • Arginase < 2.0×10-3 %,
  • NH4+ < 5.0×10-4 μg/U

Stabilizers: EDTA, glutathione, succinate, BSA

Stability: Stable at – 20 °C for at least 12 months

Molecular weight: Approx. 480,000

Isoelectric point 5.0 – 5.1

Michaelis constants: 1.05×10-2 M (Urea)

Structure: 8 active sites with SH-groups per mole of the enzyme

Inhibitors: Heavy metal ions (Ag+, Hg2+, etc.)

Optimum pH: 6.0

Optimum temperature: 60 °C

pH Stability: pH 5.5 – 8.5 (30 °C, 17hr)

Thermal stability: below 50 °C, (pH 8.0, 60min)

DIVI MANUAL.pdf

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