Description
SPECIFICATIONS:
EC 1.2.3.3
Product name: Pyruvate: oxygen 2-oxidoreductase (phosphorylating)
Appearance: Yellowish amorphous powder, lyophilized
Activity: Grade III, 1.5 U/mg-solid or more
Contaminants: ATPase: ≤ 5.0×10-2 %, GOT & GPT: ≤ 5.0×10-2 %
Stability: Stable at 2 – 8 °C for at least 12 months
Stabilizers: Sugars, FAD
Molecular weight: approx. 260,000
Isoelectric point 4.3
Michaelis constant: 3.4×10-4M (Pyruvate)
Inhibitors: Fe2+, Zn2+, Cu2+, Ag+, Hg2+
Optimum pH: 5.7
Optimum temperature: 65℃
pH Stability: pH 5.7 – 6.5 (25℃, 20hr)
Thermal stability: Below 45℃ (pH 6.0, 15min)