Description
SPECIFICATIONS:
EC 4.1.1.31
Product name: Orthophosphate: oxaloacetate carboxy-lyase
(phosphorylating)
Appearance: white amorphous powder, lyophilized
Activity: Grade III, 5.0 U/mg-solid or more
Contaminants:
- Lactate dehydrogenase: ≤ 1.0×10-3 %,
- Pyruvate kinase: ≤ 0.5 %
Stability: Stable at – 20 °C for at least 12 months
Molecular weight: Approx. 390,000 (by gel filtration)
Structure: 4 Subunits per mole of enzyme
Isoelectric point 6.0 + 0.1
Michaelis constants: 1.9×10-4 M (Phosphoenolpyruvate)
Optimum pH: 7.5 – 8.0
Optimum temperature: 60℃
pH Stability: pH 5.0 – 8.0 (25℃, 24hr)
Thermal stability: Below 40 ℃ (pH 7.0, 15min)