Description
SPECIFICATIONS
EC 1.11.1.7
Product name: Donor:hydrogen-peroxidase oxidoreductase
Appearance: Reddish brown amorphous powder lyophilized
Activity: Grade I, 250 Purpurogallin U/mg-solid or more (RZ≥ 3.0 salt free)
Stability: Stable at －20℃ for at least 12 months
Molecular weight: approx. 40,000
Structure: Glycoprotein with one mole of protohaemin Ⅸ
Inhibitors: Cyanide, sulfide, fluoride, azide
Optimum pH: 6.0－7.0
Optimum temperature: 45 ℃
pH Stability: pH 5.0－10.0 (25 ℃, 20hr)
Thermal stability below 50 ℃ (pH 6.0, 10min)
PEO 131 is chromatographically separated from the other isoenzymes using SP Sephadex C-50
by the modified method of Paul et al. The peroxidase fractions having equal RZ values (ca.3.3)
are combined to generate the preparation. This preparation has an RZ value of ca.3.3 and is
electrophoretically homogeneous.