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Malate Dehydrogenase from microorganism

Malate Dehydrogenase from microorganism

Product Code: 183654

This enzyme is used for enzymatic determination of L- malate and glutamate oxaloacetate transaminase (GOT) in clinical analysis

Product Code: 183654 Category:

Description

SPECIFICATIONS

Product Name: L-malate: NAD+ oxidoreductase

EC: 1.1.1.37

Appearance: Slightly yellowish amorphous powder, lyophilized

Activity: Grade II, 40 U/mg-solid or more

Contaminants:

  • Glutamate oxaloacetate transaminase ≤ 1.0×10-3%
  • Lactate dehydrogenase ≤ 1.0×10-3%
  • NADH oxidase ≤ 1.0×10-3%

Stability: Stable at – 20℃ for at least 12 months

Molecular weight: Approx. 140,000

Isoelectric point: pH 4.8±0.1

Michaelis constants: 

  • 5.4×10-5M (L-Malate), 5.0×10-6 M (Oxaloacetate)
  •  8.1×10-6M (NADH)

Structure: 4 subunits per mole of enzyme

Inhibitors: Hg2+

Optimum pH: 8.0

Optimum temperature: 70℃

pH Stability: pH 3.0－9.0 (25℃, 20hr)

Thermal stability: Below 70℃ (pH 7.5, 15min)

 

DIVI MANUAL.pdf