Description
SPECIFICATIONS:
EC 1.1.1.27
Product name: (S)-Lactate: NAD+ oxidoreductase
Appearance: Crystalline suspension in 1.6M ammonium sulfate solution
Activity: Grade II, 2,000 U/ml or more
Contaminants:
- Malate dehydrogenase: ≤ 5.0×10-2%
- GPT: ≤ 3.0×10-2%
- Pyruvate kinase: ≤ 3.0×10-2%
Stabilizers: NADH, 2-mercaptoethanol
Stability: Stable at 2-8 °C for at least 12 months
Molecular weight: 115,000 ± 6,500
Michaelis constants: 2.5×10-2M (Lactate), 1.0×10-4M (Pyruvate)
Structure: 4 subunits per mol of enzyme
Inhibitors: I－, Ag＋, Hg2＋, p-chloromercuribenzoate, LDH inhibitors
(formed from NADH)
Optimum pH: 6.0 – 7.4
Optimum temperature: above 60℃
pH Stability: pH 6.0−8.0 (23℃, 22hr)
Thermal stability: Below 50 ℃ (pH 7.4, 10min)