Lactate Dehydrogenase from Pig heart

Lactate Dehydrogenase from Pig heart

Product Code: 181547

EC no:     1.1.1.27

Product Code: 181547 Category:

Description

SPECIFICATIONS:

EC 1.1.1.27

Product name: (S)-Lactate: NAD+ oxidoreductase

Appearance: Crystalline suspension in 1.6M ammonium sulfate solution

Activity: Grade II, 2,000 U/ml or more

Contaminants:

  • Malate dehydrogenase: ≤ 5.0×10-2%
  • GPT: ≤ 3.0×10-2%
  • Pyruvate kinase: ≤ 3.0×10-2%

Stabilizers: NADH, 2-mercaptoethanol

Stability: Stable at 2-8 °C for at least 12 months

Molecular weight: 115,000 ± 6,500

Michaelis constants: 2.5×10-2M (Lactate), 1.0×10-4M (Pyruvate)

Structure: 4 subunits per mol of enzyme

Inhibitors: I-, Ag+, Hg2+, p-chloromercuribenzoate, LDH inhibitors
(formed from NADH)

Optimum pH: 6.0 – 7.4

Optimum temperature: above 60℃

pH Stability: pH 6.0−8.0 (23℃, 22hr)

Thermal stability: Below 50 ℃ (pH 7.4, 10min)

DIVI MANUAL.pdf

X