Description
SPECIFICATIONS
Product Name: ATP: D-Hexose 6-phosphotransferase
EC: 2.7.1.1
Appearance: White amorphous powder lyophilized
Activity: Grade III 150 U/mg-solid or more
Contaminants:
- Phosphoglucose isomerase ≤ 1.0×10-1 %
- 6-Phosphogluconate dehydrogenase ≤ 1.0×10-2 %
- Glucose-6-phosphate dehydrogenase ≤ 1.0×10-2 %
- Myokinase ≤ 1.0×10-2 %
- Glutathione reductase ≤ 1.0×10-1 %
Stability: Store at -20 ℃
Molecular weight: Approx. 82,000
Isoelectric point: pH 4.8±0.1
Michaelis constants:
- 2.3×10-4 M (D-Glucose), 7.7×10-5 M (ATP)
- 8.1×10-6M (NADH)
Inhibitors: Metal ions, p-chloromercuribenzoate, iodoacetamide, SDS, etc.
Activators: Mg2+
Optimum pH: 8.0 – 9.0
Optimum temperature: 50 ℃
pH Stability: pH 4.0 – 9.0 (25 ℃, 20hr)
Thermal stability: Below 45 ℃ (pH 7.0, 30min)