Description
SPECIFICATIONS
EC 1.1.1.6
Product name Glycerol:NAD+ 2-oxidoreductase
Appearance White amorphous powder, lyophilized
Activity Grade III, 50 U/mg-solid or more (containing 50% of stabilizers)
Contaminants NADH oxidase : ≤ 1.0×10-3 %
Stabilizers BSA
Stability Stable at －20℃ for at least 12 month
Molecular weight approx. 390,000
Isoelectric point 4.4±0.1
Michaelis constants 1.1×10-2M (Glycerol), 8.9×10-5M (NAD+)
Structure 10 subunits (42,000) per mol of enzyme
Inhibitors p-Chloromercuribenzoate, o-phenanthroline, monoiodoacetate, heavy metal ions (Co2+, Ni2+, Cu2+, Zn2+, Cd2+)
Optimum pH 10.0－10.5
Optimum temperature 50℃
pH Stability pH 7.5－10.5 (25℃, 20hr)
Thermal stability below 55℃ (pH 7.5, 15min)
Substrate specificity This enzyme has the highest specificity for glycerol and 1,2-propanediol, and also oxidizes glycerol-α-monochlorohydrin, ethylene glycol and 2,3-butanediol. The oxidative reaction is stimulated by K+, NH4+ and Rb+.