Description
SPECIFICATIONS
EC 1.4.1.2
Product name L-Glutamate:NAD+ oxidoreductase (deaminating)
Appearance White amorphous powder lyophilized
Activity Grade II, 100 U/mg-solid or more
Contaminants NAD oxidase ≤ 1.0×10-2 %
Stability Stable at －20℃ for at least 12 months
Molecular weight approx. 260,000
Isoelectric point 5.6
Michaelis constants 9.21×10-3 M (NH3), 4.80×10-3M (α-Ketoglutarate), 5.89×10-4M (NAD＋), 7.8×10-5M (L-Glutamate), 1.29×10-4M (NADH)
Structure 6 subunits per mol of enzyme
Inhibitors Heavy metals, PCMB, IAA
Optimum pH 7.5 – 8.0 (a-KG àL-Glu), 9.0 (L-Glu à a-KG)
Optimum temperature 55℃ (α-KG→L-Glu), 50℃ (L-Glu→α-KG)
pH stability 5.0 – 10.0 (25°C, 20hr)
Thermal stability below 50℃ (pH 8.3, 10min)