Description
SPECIFICATIONS:
EC 1.1.1.47
Product name: β-D-Glucose: NAD(P)+1-oxidoreductase
Appearance: White amorphous powder lyophilized
Activity: Grade III, 250U/mg-solid or more
Contaminants:
- NADH oxidase ≤ 1.0×10-3%
- α-Glucosidase ≤ 1.0×10-3%
- Glucose-6-phosphate dehydrogenase ≤ 1.0×10-3%
Stability: Stable at – 20 °C for at least 12 months
Molecular weight: Approx. 101,000 (Gel filtration)
Isoelectric point 4.5
Michaelis constants: NAD+ linked: 1.38×10-2M (D-Glucose), 3.09×10-4M (NAD+)
NADP+ linked: 1.25×10-2M (D-Glucose), 4.07×10-5M
(NADP+)
Inhibitors: Ag+, Hg2+, Monoiodoacetate
Optimum pH: 9.0
Optimum temperature: 55℃
pH Stability: pH 6.0−7.5 (20℃, 16hr)
Thermal stability: 45 °C (15 min treatment with 50mM K-Phosphate buffer, pH
7.0)
Activity: Grade III, 250U/mg-solid or more