Description
SPECIFICATIONS:
EC 1.2.1.46
Product name: Formaldehyde: NAD+ oxidoreductase
Appearance: White amorphous powder, lyophilized
Activity: Grade II, 1.0 U/mg-solid or more (contains approx. 70%
stabilizers)
Contaminants: NADH oxidase: ≤1.0×10-1 %
Stabilizers: Mg2+, Ca2+, bovine serum albumin, glycine, lysine
Stability: Stable at – 20 °C for at least one year
Molecular weight: Approx. 150,000 (by gel filtration)
Isoelectric point 5.25
Michaelis constants: 8.0×10-5 M (HCHO), 1.2×10-4 M (NAD+)
Structure: 2 subunits (75,000) per mol of enzyme
Inhibitors: Chelating agents, Ni2+, Cd2+, Hg2+, PCMB, ionic detergents
Optimum pH: 9.0
Optimum temperature: 40℃
pH Stability: pH 8.0 – 10.0 (30℃, 16hr)
Thermal stability: Below 40 ℃ (pH 7.5, 30min)