Description
SPECIFICATIONS:
EC 1.6.99
Product name: NAD(P)H: (acceptor) oxidoreductase
Appearance: Yellowish amorphous powder, lyophilized
Activity: Grade III, 30 U/mg-solid or more (containing approx. 15%
stabilizers)
Contaminants: Myokinase: ≤ 5.0×10-1%, NAD(P)H oxidase: ≤ 5.0×10-1%
Stabilizers: FMN, NAD(P)H
Stability: Stable at – 20 °C for at least one year
Molecular weight: Approx. 24,000
Michaelis constants: 2.0×10-5 M (NADH), 6.0×10-6 M (NADPH)
Structure: One mol of FMN per mol of enzyme
Inhibitors: N-Ethylmaleimide
Optimum pH: 8.5
Optimum temperature: 50℃
pH Stability: pH 7.5 (30℃, 3hr)
Thermal stability: Below 30 ℃ (pH 7.5, 30min)
Substrate specificity: Either NADH or NADPH can be used as a reductant. The
catalytic ratio (NADPH/NADH) is 0.6 in the assay method.
Neither oxygen nor cytochrome C can be utilized as a
hydrogen acceptor.