Description
SPECIFICATIONS
EC 1.1.1.30
Product name (R)-3-hydroxybutanoate: NAD+ oxidoreductase
Appearance white amorphous powder lyophilized
Activity Grade III, 100 U/mg-solid or more
Contaminants NADH oxidase ≤ 2.0×10－3%
Malate dehydrogenase ≤ 2.0×10－3%
Lactate dehydrogenase ≤ 2.0×10－3%
Stabilizers Sucrose, mannitol, BSA
Stability Stable at －20℃ for at least 12 months
Molecular weight approx. 130,000 (by gel filtration)
Isoelectric point 5.6±0.1
Michaelis constants 4.2×10-4M (25℃, pH8.3), 7.0×10-4M (37℃, pH8.3) (D-3-Hydroxybutyrate) 4.9×10-5M (25℃, pH8.3), 7.2×10-5M (37℃, pH8.3) (NAD＋) 8.1×10-5M (25℃, pH7.1), 2.4×10-4M (37℃, pH7.1) (Acetoacetate) 8.4×10-6M (25℃, pH7.1), 1.5×10-5M (37℃, pH7.1) (NADH)
Inhibitors PCMB, MIA, IAA, Ag+, Hg2+, SDS, DAC
Optimum pH 8.3
Optimum temperature 55℃
pH Stability pH 5.0－8.5 (25℃, 20hr)
Thermal stability below 40℃ (pH 6.5, 15min)