Creatininase from Microorganism

Creatininase from Microorganism

Product Code: 173609

EC:  3.5.2.10

Product Code: 173609 Category:

Description

SPECIFICATIONS

EC  3.5.2.10

Product name:                        Creatinine amidohydrolase

Appearance:                          White amorphous powder lyophilized

Activity:                                   Grade III 150 U/mg-solid or more

Contaminant:                          NADH oxidase ≤ 5.0×10-2 % Catalase ≤ 2.0 %

Stabilizers:                             Sucrose, BSA

Stability:                                 Stable at -20℃ for at least 12 months

Molecular weight:                   approx. 175,000

Isoelectric point:                     4.7

Michaelis constants:              3.2×10-2 M (Creatinine), 5.7×10-2 M (Creatine)

Structure:                               8 subunits per mol of enzyme (One mol of zinc is bound to each subunit)

Inhibitors:                              Ag+, Hg2+ N-bromosuccinimide, EDTA

Optimum pH:                        6.5-7.5

Optimum temperature:          70 ℃

pH Stability:                           pH 7.5-9.0 (5℃, 16hr)

Thermal stability:                   below 70 ℃ (pH 7.5, 30min)

DIVI MANUAL.pdf

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