Description
SPECIFICATIONS
EC 3.5.2.10
Product name: Creatinine amidohydrolase
Appearance: White amorphous powder lyophilized
Activity: Grade III 150 U/mg-solid or more
Contaminant: NADH oxidase ≤ 5.0×10-2 % Catalase ≤ 2.0 %
Stabilizers: Sucrose, BSA
Stability: Stable at －20℃ for at least 12 months
Molecular weight: approx. 175,000
Isoelectric point: 4.7
Michaelis constants: 3.2×10-2 M (Creatinine), 5.7×10-2 M (Creatine)
Structure: 8 subunits per mol of enzyme (One mol of zinc is bound to each subunit)
Inhibitors: Ag+, Hg2+ N-bromosuccinimide, EDTA
Optimum pH: 6.5－7.5
Optimum temperature: 70 ℃
pH Stability: pH 7.5－9.0 (5℃, 16hr)
Thermal stability: below 70 ℃ (pH 7.5, 30min)