Description
SPECIFICATIONS
EC 3.5.4.21
Product name: Creatinine iminohydrolase
Appearance: White amorphous powder lyophilized
Activity: Grade III, 10U/mg-solid or more (containing ~ 30% of stabilizer)
Contaminants: Creatinine amidohydrolase ≤ 1.0×10-2 % Creatine amidinohydrolase ≤ 1.0×10-2 % Urease ≤ 1.0×10-2 % NADH oxidase ≤ 1.0×10-2 % NH4+ ≤ 1.0×10-2 μg/u
Stabilizers: Mannitol
Stability: Stable at －20℃ for at least 12 months
Molecular weight: approx. 260,000
Isoelectric point: 4.4
Michaelis constant: 3.5×10-3M (Creatinine)
Structure: 6 subunits per mol of enzyme
Inhibitors: Ag＋,Hg2＋, o-phenanthroline,monoiodoacetate
Optimum pH: 8.5－9.5
Optimum temperature: 65－75℃
pH Stability: pH 7.0－11.0 (30℃, 20hr)
Thermal stability: below 65℃ (pH 7.5, 1hr)