Description
SPECIFICATIONS:
EC 3.5.3.3
Product name: Creatine amidinohydrolase
Appearance: White amorphous powder lyophilized
Activity: Grade II, 4.0 U/mg-solid or more
Contaminants: NADH oxidase ≤ 5.0×10-2 %, Catalase ≤ 2.0%
Stabilizers: Sugars, EDTA
Stability: Stable at – 20 °C for at least one year
Molecular weight: Approx. 67,000 by gel filtration
Isoelectric point 4.5 ± 0.1
Michaelis constants: 4.5×10-3 M (Creatine)
Inhibitors: Hg2+, Cu2+, Ag+, SH reagent (NEM), PCMB
Optimum pH: 6.5 – 7.5
Optimum temperature: 40 – 50℃
pH Stability: pH 4.0 – 10.0 (25 ℃, 20hr)
Thermal stability: Below 50 ℃ (pH 7.5, 30min)