Description
SPECIFICATIONS
EC 1.1.3.6
Product name: Cholesterol: oxygen oxidoreductase
Appearance: Yellowish amorphous powder, lyophilized
Activity: Grade III, 15 U/mg or more (contains approx. 40%
stabilizers)
Contaminants: Catalase ≤ 0.1 %, Cholesterol esterase: < 0.01 %
Stabilizers: Bovine serum albumin, amino acids
Stability: Stable at – 20 °C for at least one year
Molecular weight: Approx. 34,000 (by gel-filtration)
Isoelectric point: 5.1 ± 0.1 and 5.4 ± 0.1
Michaelis constants: 4.3 x 10-5 M (Cholesterol)
Inhibitors: Ionic detergents, Ag+, Hg2+
Optimum pH: 6.5 – 7.0
Optimum temperature: 45 – 50 ℃
pH Stability: pH 5.0 – 10.0 (25 ℃, 20hr)
Thermal stability: Below 45 ℃ (pH 7.0, 15min)