Description
SPECIFICATIONS
EC 1.1.3.6
Product name: Cholesterol: oxygen oxidoreductase
Appearance: Yellowish amorphous powder, lyophilized
Activity: Grade III, 12 U/mg or more
Contaminant:
- Catalase: < 0.1 %
- Cholesterol esterase: < 0.01 %
Stabilizers: Bovine serum albumin, amino acids
Stability: Stable at – 20 ℃ for at least 12 months
Molecular weight: Approx. 55000 (by gel-filtration)
Isoelectric point: 4.6 ± 0.1 and 4.9 ± 0.1 and 5.2 ± 0.1
Michaelis constants: 2.1 x 10-5M (Cholesterol)
Inhibitors: Ionic detergents, Ag+, Hg2+
Optimum pH: 7.0 – 8.0
Optimum temperature: 60 ℃
pH Stability: pH 5.0 – 10.0 (25 °C, 20hr)
Thermal stability: below 55 ℃ (pH 7.0, 15min)