Cholesterol Esterase from Pseudomonas sp.

Cholesterol Esterase from Pseudomonas sp.

Product Code: 172612

EC:  3.1.1.13

Product Code: 172612 Category:

Description

SPECIFICATIONS

EC 3.1.1.13

Product name: Steryl-ester acylhydrolase

Appearance: Light brown amorphous powder lyophilized

Activity: Grade III, 100 U/mg-solid or more (containing
approx. 40% of stabilizers)

Contaminant: Catalase ≤ 1.0×10-2%

Stabilizers: Mg2+, Na-cholate, bovine serum albumin

Stability: Stable at – 20 ℃ for at least 12 months

Molecular weight: approx. 300,000

Isoelectric point: 5.9 ± 0.1

Michaelis constants:

  • 5.4×10-5M (Linoleate), 6.6×10-5 M (Oleate)
  • 3.7×10-5M (Linolenate), 1.5×10-4 M (Palmitate)
  • 1.2×10-4M (Myristate), 2.3×10-5 M (Stearate)

Inhibitors: Hg2+, Ag+, ionic detergents

Optimum pH: 7.0 – 9.0

Optimum temperature: 40 ℃

pH Stability: 5.0 – 9.0 (25 °C, 24hr)

Thermal stability: below 55 ℃ (pH 7.5, 10min)

DIVI MANUAL.pdf

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