Ascorbate oxidase from Cucurbita sp

Ascorbate oxidase from Cucurbita sp

Product Code: 194604

EC:  1.10.3.3

Product Code: 194604 Category:

Description

SPECIFICATIONS

EC  1.10.3.3

Product name: L-Ascorbate: oxygen oxidoreductase

Appearance: Light blue amorphous powder, lyophilized

Activity: Grade III, 200 U/mg solid or more

Contaminants: Catalase:< 0.1 % Phosphatase:< 0.02%

Stability: Stable at -20°C for at least 12 months

Stabilizers: Bovine serum albumin, borax, basic amino acids

Molecular weight: 140,000

Isoelectric point: 5.5 ± 0.2

Michaelis constant: 2.7 x 10-4 M (Ascorbate)

Inhibitors: Cyanide, Na2S, diethyldithiocarbamate (Na)

Optimum pH: 5.5 – 6.5

Optimum temperature: approx. 45 °C

pH Stability: pH 6.0 – 10.0 (25 °C, 20hr)

Thermal stability: Below 45 °C (pH 7.0, 30 min)

Substrate specificity: The enzyme oxidizes ascorbic acid and several ascorbic acid derivatives.

DIVI MANUAL.pdf

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