Malate Dehydrogenase from microorganism

Malate Dehydrogenase from microorganism

Product Code: 183654

This enzyme is used for enzymatic determination of L- malate and glutamate oxaloacetate transaminase (GOT) in clinical analysis

Product Code: 183654 Category:

Description

SPECIFICATIONS

Product NameL-malate: NAD+ oxidoreductase

EC1.1.1.37

AppearanceSlightly yellowish amorphous powder, lyophilized

ActivityGrade II, 40 U/mg-solid or more

Contaminants:

  • Glutamate oxaloacetate transaminase ≤ 1.0×10-3%
  • Lactate dehydrogenase ≤ 1.0×10-3%
  • NADH oxidase ≤ 1.0×10-3%

StabilityStable at – 20for at least 12 months

Molecular weightApprox. 140,000

Isoelectric pointpH 4.8±0.1

Michaelis constants

  • 5.4×10-5M (L-Malate), 5.0×10-6 M (Oxaloacetate)
  •  8.1×10-6M (NADH)

Structure4 subunits per mole of enzyme

InhibitorsHg2+

Optimum pH8.0

Optimum temperature70

pH StabilitypH 3.09.0 (25, 20hr)

Thermal stabilityBelow 70(pH 7.5, 15min)

 

DIVI MANUAL.pdf

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