Description
SPECIFICATIONS
EC 1.4.1.4
Product name: L-Glutamate:NADP+ oxidoreductase (deaminating)
Appearance 50mM Tris buffer solution at pH 7.8 with 0.05% NaN3 and 5mM EDTA
Activity > 9000 U/ml
Contaminants NADPH oxidase ≤ 0.01 %, Gluthathione reductase ≤ 0.01 %
Stabilizer EDTA
Stability Stable at 2-8°C for at least 6 months
Molecular weight Approx. 300,000
Isoelectric point 4.6
Michaelis constants 1.1×10-3 M (NH3), 3.4×10-4 M (α-Ketoglutarate), 1.5×10-5M (NADP+) 1.2×10-3 M (L-Glutamate), 1.4×10-5M (NADPH)
Structure 6 subunits per mol of enzyme
Inhibitors Heavy metals, PCMB, Pyridine, 4-4’-dithiopyridine, 2-2’-dithiopyridine
Optimum pH 8.5 (a-KGàL-Glu), 9.8 (L-Gluàa-KG)
Optimum temperature 45°C (a-KGàL-Glu), 45-55°C (L-Gluàa-KG)
pH stability 6.0 – 8.5 (25°C, 20hr)
Thermal stability Below 50°C (pH 7.4, 10min)