Description
SPECIFICATIONS
EC 4.1.3.3
Product name: N-Acetylneuraminate pyruvate-lyase
Appearance: Yellowish amorphous powder, lyophilized
Activity: Grade III, 15 U/mg-solid or more (contains approx. 30% stabilizers)
Contaminants: Catalase : ≤ 1.0%, NADH oxidase : ≤ 1.0×10-3%
Stabilizers: Mannitol, EDTA
Stability: Stable at – 20℃ for at least 12 months
Molecular weight: approx. 98,000
Isoelectric point: 4.6 ± 0.1
Michaelis constant: 2.5×10-3 M (N-Acetylneuraminic acid)
Structure: 3 subunits per mol of enzyme
Inhibitors: p-Chloromercuribenzoate, sodium dodecyl sulfate, Hg++,Ag+
Optimum pH: 7.5 – 8.0
Optimum temperature: 70℃
pH Stability: pH 6.0 – 9.0 (10℃, 25hr)
Thermal stability: below 65℃ (pH 7.5, 30min