Description
SPECIFICATIONS
EC 2.7.1.30
Product name ATP: Glycerol 3-phosphotransferase
Appearance: White amorphous powder lyophilized
Activity: Grade III, 20 U/mg-solid or more (contains approx. 50% stabilizers)
Contaminants: Catalase : ≤ 1.0×10-1 %,
NADH oxidase : ≤ 1.0×10-2 %
Phosphatase (pH 6.0) : ≤ 2.0×10-3 %
Stability: Stable at -20°C for at least 12 months
Molecular weight: approx. 128,000 (by gel filtration)
Structure: 4 subunits of approx 58,000
Isoelectric point: 4.2
Michaelis constants: 4.4×10-5 M (Glycerol), 4.3×10-4 M (ATP)
Inhibitors: p-Chloromercuribenzoate, Hg++, Ag+, Pb++, Fe++
Optimum pH: 9.8 (G-3-PDH system), 7.8 (G-3-P oxidase system)
Optimum temperature: 50℃
pH Stability: pH 5.5 – 10.0 (25°C, 20hr)
Thermal stability: below 40℃ (pH 7.5, 15min)