Description
SPECIFICATIONS
EC 1.1.99.17
Name D-Glucose:(flavine adenine dinucleotide)-dehydrogenase
Appearance Yellowish amorphous powder lyophilized
Activity Grade III, 500U/mg-solid or more
Contaminants Glucose dehydrogenase < 2.0×10-2 % (NAD-dependent) Hexokinase < 2.0×10-2 %, Glucose oxidase < 2.0×10-2 % Glucoamylase < 1.0×10-2 %
Stability Stable at – 20°C for at least 12 months
Molecular weight approx. 100,000 (by gel filtration）
Michaelis constant 4.8 mM (D-Glucose)
Inhibitors Cu2+, Pb2+, Ag+
Optimum pH 7.0
Optimum temperature 47°C
pH Stability 3.5 – 8.5 (25°C, 16hr)
Temperature stability below 50°C (pH 7.5, 30min)