Description
SPECIFICATIONS:
EC 2.4.2.1
Product name: Purine-nucleoside: orthophosphate ribosyltransferase
Appearance: White amorphous powder lyophilized
Activity: Grade III, 15 U/mg-solid or more
Contaminants:
- Catalase: < 20%, 5′-Nucleosidase ≤ 1.0×10-3 %,
- Adenosine deaminase ≤ 1.0×10-3 %, ATPase ≤ 1.0×10-2 %
Stabilizers: K-Gluconate, mannitol, EDTA
Stability: Stable at – 20 °C for at least 12 months
Molecular weight: Approx. 120,000
Isoelectric point 4.1±0.1
Michaelis constants: 6.4×10-5 M (Inosine), 3.2×10-4 M (Pi)
Inhibitors: Chloromercuribenzoate, SDS, Hg2+, Ag+
Optimum pH: 7.5 – 8.0
Optimum temperature: 65 ℃
pH Stability: pH 6.0−9.0 (30℃, 16hr)
Thermal stability: Below 60 ℃ (pH 7.7, 30min)