N-Acetylneuraminic acid aldolase from microorganism

N-Acetylneuraminic acid aldolase from Microorganism

Product Code: 141301

EC:  4.1.3.3

Product Code: 141301 Category:

Description

SPECIFICATIONS

EC  4.1.3.3

Product name:    N-Acetylneuraminate pyruvate-lyase

Appearance:  Yellowish amorphous powder, lyophilized

Activity:   Grade III, 15 U/mg-solid or more (contains approx. 30% stabilizers)

Contaminants:   Catalase : ≤ 1.0%, NADH oxidase : ≤ 1.0×10-3%

Stabilizers:   Mannitol, EDTA

Stability:   Stable at – 20℃ for at least 12 months

Molecular weight:   approx. 98,000

Isoelectric point:   4.6 ± 0.1

Michaelis constant:   2.5×10-3 M (N-Acetylneuraminic acid)

Structure:   3 subunits per mol of enzyme

Inhibitors:   p-Chloromercuribenzoate, sodium dodecyl sulfate, Hg++,Ag+

Optimum pH:   7.5 – 8.0

Optimum temperature:   70℃

pH Stability:  pH 6.0 – 9.0 (10℃, 25hr)

Thermal stability:  below 65℃ (pH 7.5, 30min

DIVI MANUAL.pdf

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