Glycerol kinase from Cellulomonas sp

Glycerol kinase from Cellulomonas sp

Product Code: 184534

EC:  2.7.1.30

Product Code: 184534 Category:

Description

SPECIFICATIONS

EC  2.7.1.30

Product name ATP:      Glycerol 3-phosphotransferase

Appearance:     White amorphous powder lyophilized

Activity:   Grade III, 20 U/mg-solid or more (contains approx. 50% stabilizers)

Contaminants:   Catalase : ≤ 1.0×10-1 %,

NADH oxidase : ≤ 1.0×10-2 %

Phosphatase (pH 6.0) : ≤ 2.0×10-3 %

Stability:     Stable at -20°C for at least 12 months

Molecular weight:     approx. 128,000 (by gel filtration)

Structure:    4 subunits of approx 58,000

Isoelectric point:    4.2

Michaelis constants:        4.4×10-5 M (Glycerol), 4.3×10-4 M (ATP)

Inhibitors:   p-Chloromercuribenzoate, Hg++, Ag+, Pb++, Fe++

Optimum pH:   9.8 (G-3-PDH system), 7.8 (G-3-P oxidase system)

Optimum temperature:   50℃

pH Stability:   pH 5.5 – 10.0 (25°C, 20hr)

Thermal stability:   below 40℃ (pH 7.5, 15min)

DIVI MANUAL.pdf

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