Glutamate dehydrogenase (NAD dependent) from Microorganism

Product Code: 183636

EC:  1.4.1.2



SKU: 183636

Category:

Product Description

SPECIFICATIONS

EC  1.4.1.2

Product name L-Glutamate:NAD+ oxidoreductase (deaminating)

Appearance White amorphous powder lyophilized

Activity Grade II, 100 U/mg-solid or more

Contaminants NAD oxidase ≤ 1.0×10-2 %

Stability Stable at -20℃ for at least 12 months

Molecular weight approx. 260,000

Isoelectric point 5.6

Michaelis constants 9.21×10-3 M (NH3), 4.80×10-3M (α-Ketoglutarate), 5.89×10-4M (NAD+), 7.8×10-5M (L-Glutamate), 1.29×10-4M (NADH)

Structure 6 subunits per mol of enzyme

Inhibitors Heavy metals, PCMB, IAA

Optimum pH 7.5 - 8.0 (a-KG àL-Glu), 9.0 (L-Glu à a-KG)

Optimum temperature 55℃ (α-KG→L-Glu), 50℃ (L-Glu→α-KG)

pH stability 5.0 - 10.0 (25°C, 20hr)

Thermal stability below 50℃ (pH 8.3, 10min)

Glutamate dehydrogenase (NAD dependent) from Microorganism, Activity:>100U/mg - Innovative Enzymes Glutamate dehydrogenase (NAD dependent) from Microorganism, Activity:>100U/mg - Innovative Enzymes

Glutamate dehydrogenase (NAD dependent) from Microorganism, Activity:>100U/mg

Glutamate dehydrogenase (NAD dependent) from Microorganism

Product Code: 183636

EC:  1.4.1.2

Product Code: 183636 Category:

Description

SPECIFICATIONS

EC  1.4.1.2

Product name L-Glutamate:NAD+ oxidoreductase (deaminating)

Appearance White amorphous powder lyophilized

Activity Grade II, 100 U/mg-solid or more

Contaminants NAD oxidase ≤ 1.0×10-2 %

Stability Stable at -20℃ for at least 12 months

Molecular weight approx. 260,000

Isoelectric point 5.6

Michaelis constants 9.21×10-3 M (NH3), 4.80×10-3M (α-Ketoglutarate), 5.89×10-4M (NAD+), 7.8×10-5M (L-Glutamate), 1.29×10-4M (NADH)

Structure 6 subunits per mol of enzyme

Inhibitors Heavy metals, PCMB, IAA

Optimum pH 7.5 – 8.0 (a-KG àL-Glu), 9.0 (L-Glu à a-KG)

Optimum temperature 55℃ (α-KG→L-Glu), 50℃ (L-Glu→α-KG)

pH stability 5.0 – 10.0 (25°C, 20hr)

Thermal stability below 50℃ (pH 8.3, 10min)

DIVI MANUAL.pdf

X