Glucose-6-Phosphate Dehydrogenase from Microorganism

Glucose-6-Phosphate Dehydrogenase from Microorganism

Product Code: 183626

EC no:     1.1.1.49

Product Code: 183626 Category:

Description

SPECIFICATIONS:

EC 1.1.1.49

Product name: D-Glucose-6-phosphate: NADP+ 1-oxydoreductase

Appearance: white amorphous powder lyophilized

Activity: Grade III, 200 U/mg-solid or more

Contaminants:

  • Creatine phosphokinase ≤ 1×10-3%
  • Phosphoglucomutase ≤ 1×10-3%
  • 6-Phosphogluconate dehydrogenase ≤ 5×10-3%
  • Phosphoglucose isomerase ≤ 1×10-2%
  • Glutathione reductase ≤ 1×10-3%
  • Hexokinase ≤ 1×10-2%, Myokinase ≤ 1×10-2%
  • NADH oxidase ≤ 1×10-2%, NADPH oxidase ≤ 1×10-2%

Stability: Stable at – 20 °C for at least 12 months

Molecular weight: 140,000 (Gel filtration)

Michaelis constants: NAD+ linked: 2.4×10-4M (NAD+), 4.7×10-4M (G6P)
NADP+ linked: 7.4×10-6M (NADP+), 3.2×10-4M (G6P)

Inhibitors: Metal ions, Iodoacetamimide, SDS etc.

Optimum pH: 7.8

Optimum temperature: 50 – 55℃

pH Stability: pH 5−11 (25℃, 22hr)

Thermal stability: Below 50 ℃ (pH 7.8, 30min)

DIVI MANUAL.pdf

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