Ascorbate oxidase from Cucurbita sp Product Code: 194604 EC: 1.10.3.3 SKU: 194604 Category: Enzymes |
Product Description |
SPECIFICATIONSEC 1.10.3.3 |
Product name: L-Ascorbate: oxygen oxidoreductase |
Appearance: Light blue amorphous powder, lyophilized |
Activity: Grade III, 200 U/mg solid or more |
Contaminants: Catalase:< 0.1 % Phosphatase:< 0.02% |
Stability: Stable at -20°C for at least 12 months |
Stabilizers: Bovine serum albumin, borax, basic amino acids |
Molecular weight: 140,000 |
Isoelectric point: 5.5 ± 0.2 |
Michaelis constant: 2.7 x 10-4 M (Ascorbate) |
Inhibitors: Cyanide, Na2S, diethyldithiocarbamate (Na) |
Optimum pH: 5.5 - 6.5 |
Optimum temperature: approx. 45 °C |
pH Stability: pH 6.0 - 10.0 (25 °C, 20hr) |
Thermal stability: Below 45 °C (pH 7.0, 30 min) |
Substrate specificity: The enzyme oxidizes ascorbic acid and several ascorbic acid derivatives. |