This chromogen is used for the detection of Fe(II) and Co(III) in clinical chemistry reagents. EDTA can dissolve Cu(II) and Ni(II) cmplexes so it is useful to determine Fe in the presence of Cu and Ni. Fe(II) complexe absorbs at 558nm / 89,000.
Appearance: Reddish orange or orange powder
Molecular weight: 478.34
Molecular formula: C17H21BrN5NaO3S
Absorbance: > 0.40 (458 nm) (1.03 x 10-5 M in buffer)
Molar absorptivity: > 86 000 (558 nm) (Fe++ complex)
Solubility: 5 mg/10 ml water
Storage: 2 – 8 °C. Keep tightly closed. Protect from metal. Store in a dry place.
Stability: At least one year at 2 – 8 °C.